Figure 4.
Using SB-induced tumorigenesis to derive clonal relationships between primary and metastatic derivatives. (a) Heat-map showing the clonal relationship between lung metastases and HCC samples. Heat-map was generated by mapping insertion sites from 32 lung metastases nodules and 3 HCC nodules taken from the same experimental mouse (ATRP M232). Importantly, 3 additional insertion mutations were common in most of the metastases, indicating potential genes involved with the metastatic process. Red, insertion detected at the indicated locus; Black, no insertion detected at the indicated locus. (b) Phylogenic tree generated from the insertion sites of the lung metastases and HCCs.