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. Author manuscript; available in PMC: 2009 Jul 20.
Published in final edited form as: Comp Biochem Physiol A Mol Integr Physiol. 2007 Dec 23;149(3):255–266. doi: 10.1016/j.cbpa.2007.12.005

Figure 1. Overexpression of Rv1739c increases sulfate uptake.

Figure 1

A. Schematic of Rv1739c polypeptide from M. tb. The hypothetical disposition of the transmembrane spans is based on hydropathy analysis described by (Felce et al. 2004). B. Left: Anti-6His immunoblot of clarified SDS lysate of uninduced (lane 1) and IPTG-induced Tuner(DE3)pLacI cells (lane 2). Right: Coomassie Blue R250-stained gel of Rv1739c eluted from Ni-NTA column by 250 mM imidazole. C. 35S-sulfate uptake in uninduced Tuner cells or cells induced to express either M. Tb. Rv1739c (left bars) or E. coli ychM (right bars). Number of measurements is indicated within parentheses. *, p < 0.05. D. Anti-6His immunoblot of clarified SDS lysate of uninduced Tuner cells (lane 1) or Tuner cells induced to express ychM polypeptide (lane 2).