Table 1. Mass spectrometry analysis of PABPN1-interacting proteins.
NAME | MW | ACCESSION# | PEPTIDES | |||
Myoblasts | Myotubes | |||||
wt | exp | wt | exp | |||
PRMT1 | 39 | Q8C2D7 | 5 | 18 | 4 | 17 |
PRMT3 | 59 | Q922H1 | 6 | 14 | 3 | 10 |
HSP70 1A | 70 | Q61696 | 3 | 21 | 5 | 19 |
HSP70 1B | 70 | P17879 | 2 | 15 | 6 | 14 |
Hsc73 | 70 | JC4853 | 4 | 11 | 3 | 9 |
HSP70 1L | 70 | I49761 | 1 | 5 | 2 | 8 |
HSP90 alpha | 84 | P07901 | 4 | 11 | 6 | 16 |
HSP90 beta | 83 | P11499 | 4 | 10 | 7 | 19 |
Laminin receptor 1 | 32 | Q8BNL2 | 1 | 1 | 2 | 1 |
CREB-binding protein | 264 | Q8QZV8 | 1 | 1 | 0 | 0 |
N-RAP | 133 | T37192 | 0 | 0 | 2 | 1 |
YBOX1 | 35 | P62960 | 3 | 4 | 4 | 3 |
MYB-1 A | 36 | Q60950 | 2 | 5 | 6 | 4 |
MYB-1 B | 35 | Q60951 | 2 | 2 | 4 | 3 |
EF2 | 95 | P58252 | 5 | 4 | 2 | 3 |
DDEF2 | 106 | Q7SIG6 | 1 | 1 | 3 | 2 |
EF1A1 | 50 | P10126 | 7 | 9 | 3 | 3 |
Gspt1 | 49 | Q8BPH0 | 0 | 1 | 1 | 1 |
hnRNP U | 87 | O88568 | 9 | 8 | 7 | 8 |
IMPORTIN 7 | 119 | Q9EPL8 | 3 | 3 | 2 | 1 |
PABPN1 | 32 | Q8CCS6 | 6 | 5 | 6 | 6 |
SkiP | 61 | Q9CSN1 | 3 | 4 | 7 | 8 |
The number of unique peptides identified associated with normal (wt) PABPN1 is compared with the number of unique peptides identified associated with expanded (exp) PABPN1 in undifferentiated (myoblasts) and differentiated (myotubes) C2 cells. Accession # indicates UniProt accession numbers. MW indicates the calculated molecular weight in kilodaltons.