Figure 2. Akt hyper-phosphorylation does not result in increased phosphorylation of its downstream molecules.

A. mTOR total protein and phosphorylation of mTOR at Ser2448 in 6-, 27-, 33-month control (33C) and acetaminophen-treated (33T) rats as determined by immunoblotting. B. GSK3β total protein and phosphorylated GSK3β (Ser9). Data are mean±SE (n = 4). abc: Groups without the same letter are significantly different (P<0.05).