Figure 3. Akt hyper-phosphorylation does not appear to be related to increased IR-β, PI3K-p85, and PDK1 protein levels.
A. IR-β and PI3K-p85 protein in 6-, 27-, 33-month control (33C) and acetaminophen-treated (33T) rats were determined by immunoblotting. B. PDK1 total protein and phosphorylation of PDK1 at Ser241 as determined by immunoblotting. Data are mean±SE (n = 4–6). abc: Groups without the same letter are significantly different (P<0.05).