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. 2009 Jul 29;4(7):e6430. doi: 10.1371/journal.pone.0006430

Figure 4. Akt dysfunction in the very aged soleus muscle can be corrected by acetaminophen.

Figure 4

A. Akt kinase activity as measured in soleus muscles from 6-, 27-, 33-month control (33C) and acetaminophen-treated (33T) rats. The relative abundance of GSK3 fusion protein phosphorylated by the immunoprecipitated Akt was normalized to total Akt. Data are mean±SE (n = 3). abc: Groups without the same letter are significantly different (P<0.05). B. Soleus muscles from acetaminophen-treated (33T) and age-matched control (33C) animals were ex vivo incubated with insulin (+). Phosphorylation of GSK3α (Ser21), GSK3β (Ser9) and Akt (Ser473 and Thr308) after ex vivo insulin incubation were detected by immunoblotting. Muscle incubated with Krebs Henseleit buffer (−) was used as control of insulin response.