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. 2009 Apr 28;58(8):1826–1834. doi: 10.2337/db09-0132

FIG. 5.

FIG. 5.

Enhanced lipolysis is required for enhanced secretion in PKCεKO islets. Islets isolated from 12-week-old male mice were cultured for 48–72 h in the presence of 0.4 mmol/l palmitate coupled to 0.92% BSA (Palm) or BSA alone (Cont). A: Lipolysis was measured in islets, after palmitate culture, by detection of glycerol release in response to 20 mmol/l d-glucose (n = 7). B: GSIS in palmitate-cultured PKCεKO islets in the presence of the lipase inhibitor orlistat (0.2 mmol/l) or DMSO vehicle control (n = 9). C: Glucose-amplified insulin secretion measured under KATP channel–independent depolarizing conditions (25 mmol/l KCl and 100 μmol/l diazoxide) using palmitate-cultured PKCεKO islets in the presence of orlistat or DMSO control (n = 9). Data are the means ± SE. *P < 0.05; **P < 0.01. WT, wild type.