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. 2009 Apr 28;58(8):1816–1825. doi: 10.2337/db09-0063

FIG. 5.

FIG. 5.

GLP-1–increased Akt phosphorylation depends on secreted IGF-2. A: MIN6 cells were transfected with an unrelated (Un) or an IGF-2–specific shRNA. Forty-eight hours later, they were stimulated with GLP-1 for 18 h to increase IGF-1R expression and then incubated with 2 mmol/l glucose for 2 h and for 1 h in either 2 or 20 mmol/l glucose. B: MIN6 cells were stimulated with GLP-1 for 18 h and then incubated with 2 mmol/l glucose for 2 h and for 1 h in either 2 or 20 mmol/l glucose and with nonspecific IgGs or an IGF-2 blocking antibody. C: Pancreatic islets were stimulated with GLP-1 for 18 h and then incubated with 2 mmol/l glucose for 2 h and for 1 h in either 2 or 20 mmol/l glucose and with nonspecific IgGs or an IGF-2 blocking antibody. A–C: Data are means ± SD, n = 3 independent experiments. **P < 0.01; ***P < 0.001. a.u., arbitrary units.