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. 2009 Jun 9;58(8):1844–1851. doi: 10.2337/db08-0628

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 3 — Regeneration of zebrafish islets after STZ treatment. A: Hematoxylin and eosin (H&E) staining of paraffin sections at 3, 7, and 14 days. Vehicle and 14 day STZ: 200× magnification; 3 day and 7 day: 400× magnification. Arrows: islets. Arrowheads: blood vessels. B: STZ Ins/PCNA: insulin antibodies (visualized with red fluorescent secondary antibodies) mark β-cells. PCNA⁺ dividing cells are green. Arrows identify islets. Arrowheads: ducts; vehicle: numerous dividing PCNA⁺ cells are located at the base of intestinal villi. A few non–insulin expressing dividing cells are scattered throughout the islet and surrounding exocrine pancreas (400× magnification); 3 day STZ: a mantle of PCNA-positive cells surrounds affected islets (400×); 7 day STZ: dividing cells are located in and around ducts (400× magnification); inset (200× magnification): CK18 (red) labeling of ducts. Insulin⁺ cells are green; 14 day STZ: 200× magnification. Large islets with scattered dividing cells have appearance similar to vehicle-injected zebrafish. Dividing cells surround insulin-negative areas, similar to the PCNA expression observed after 3 days. C: STZ glucagon/GFP; vehicle (400× magnification): β-cells (green) and α-cells (red). Arrow: islet; 3 day STZ: glucagon⁺ cells within islet remnant (400×); 7 day STZ: glucagon labels GFP-negative islet attached to duct (200×). Inset (400×): glucagon (red) outlines islets ± β-cells (green); 14 day STZ: ductal hyperplasia (200×) with glucagon staining (red). Inset (600×): the ductal epithelium is continuous with the islet (confocal image). (A high-quality digital representation of this figure is available in the online issue.)