Fig. 2.

Flow cytometry analysis of efficient class I and green fluorescent protein (GFP) co-transfection in Cos-7 cells. Co-transfected Cos-7 cells were stained with antibody that binds both the D^b and K^b class I molecule. Shown are a representative well of (A) cells transfected with empty pcDNA 3.1 His A vector, (B) D^b plus GFP expression vectors, and (C) K^b plus GFP expression vectors. Note that cells within the upper right quadrant denotes successful co-expression of GFP and class I molecules.