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. Author manuscript; available in PMC: 2010 Mar 11.
Published in final edited form as: Structure. 2009 Mar 11;17(3):345–351. doi: 10.1016/j.str.2009.01.004

Figure 2.

Figure 2

Surfactant optimization. 15N-HSQC spectra of KcsAΔC35 solubilized in four different reverse micelle surfactant systems. Detergents were tested as aqueous samples first before preparation as reverse micelles. All reverse micelle samples were ~0.15 mM in KcsAΔC35, optimized water loadings and solubilized in pentane. Spectra were obtained at 25 °C. Panel A: 200 mM LDAO reverse micelles in pentane with 320 mM hexanol. B: 195 mM AOT and 195 mM DTAB. C: 200 mM CTAB reverse micelles in pentane with 800 mM hexanol. E: 125 mM CTAB and 125 mM DHAB with 390 mM hexanol. Insets show the spectral region containing the Trp indole N-H correlations. Panel D shows the 15N-HSQC spectrum of KcsAΔC35 solubilized in 100 mM CTAB aqueous buffer.