Fig 5.

The IFABP-TRDN-GFP transgene dramatically inhibits remodeling of multiple intestine cell types during spontaneous metamorphosis. A) Wild-type NF65 froglet; B), a sibling transgenic for IFABP-TRDN-GFP; C) entire gastro-intestinal tracts from the froglets pictured in A and B removed two weeks after the onset of feeding and completion of metamorphosis (NF66+); wild-type (upper), transgenic (lower); D,E) cross-sections from the duodenums and F,G) from the illeums of the D,F) wild-type and E,G) transgenic froglet guts in C. Solid black lines in C denote cross-section regions of the duodenums shown in D and E, and dashed lines denote cross-section regions of the ileums shown in F and G. Tissues are stained with hematoxylin and eosin. Scale bar in A denotes 1.0 mm length; scale bar in C denotes 3.0 mm length. Scale bar in E denotes 0.2 mm length.