Table 1. Relative deconjugation efficacy of different substrates in culture media.

Metabolite-containing conditioned media were obtained by incubation of γ-T (50 μM) with A549 cells for 48 h. The metabolites from culture media were extracted by ethylacetate (Materials and Methods) and were incubated with Type H-1 sulfatase (S9626 at 0.2 mg per sample) in 0.1 M NaAc (pH 5.0) at 37 °C for the indicated times. Metabolites were quantified by HPLC. Deconjugation % = 100 × (Ci-Ca)/Ci, where Ci and Ca are the concentrations of conjugated metabolites before (Ci) and after (Ca) enzyme hydrolysis. 9′S, 11′S and 13′S stand for sulfated 9′-, 11′- and 13′-carboxychromanol.

Deconjugation (%)	9′S	11′S	13′S
45 min	63 ± 1	51 ± 1	32 ± 10
90 min	79 ± 2	70 ± 2	47 ± 5