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. Author manuscript; available in PMC: 2009 Jul 20.
Published in final edited form as: J Mol Biol. 2007 Jun 4;371(2):514–527. doi: 10.1016/j.jmb.2007.05.095

Figure 5. Rad1 protein level was not elevated by the inhibitors of CSN.

Figure 5

Figure 5

(a) Curcumin and emodin did not affect 9-1-1 levels in PANC-1 cells analyzed by immunohistochemistry. PANC-1 cells were treated with vehicle control, 10μM curcumin or 20μM emodin. The expression of p27 and Rad1 were detected by immunohistochemistrical analysis. The brown color represents positive signal. (b) Curcumin and emodin did not affect 9-1-1 levels in PANC-1 cells analyzed by Western blot. PANC-1 cells were infected with retroviruses containing siGFP (Control, lane 1, 3, 5 and 7) or siJab1-1 (lane 4). Cells were then treated with vehicle control (lane 1 and 4), 10μM curcumin (lane 2) or 20μM emodin (lane 3). The expression level of Rad1, Hus1, Rad9, p27 and β-actin were detected by Western blotting using specific antibodies. (c) Curcumin and emodin changed the stability of c-Jun and p53, but did not affect 9-1-1 levels in HeLa cells. Cells were treated with vehicle control (lane 1 and 3), 10μM curcumin (lane 2) or 20μM emodin (lane 4). The expression level of c-Jun, p53, Rad1, Hus1, Rad9 and β-actin were detected by Western blotting using specific antibodies.