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. 2009 Jun 29;185(7):1275–1284. doi: 10.1083/jcb.200810002

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© 2009 Kong et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Binding specificity. (A and B) α₅β₁-Fc data are shown. (C and D) trα₅β₁-Fc data are shown. Frequencies of adhesion between 10 µg/ml FNIII_7–10 adsorbed on cantilever tips and 10 µg/ml α₅β₁-Fc (A) or trα₅β₁-Fc (C) captured by 15 or 100 µg/ml GG-7 Fab precoated on Petri dishes in buffer containing the indicated cations without or with cyclo(-GRGDSP) or HFN7.1 in solution were enumerated in 100 tests for each spot and presented as mean ± SEM of 3–5 spots. Frequencies (measured with the same protocol as in A and C) of adhesion between 10 µg/ml FNIII_7–10 coated on cantilever tips and 10 µg/ml α₅β₁-Fc (B) or trα₅β₁-Fc (D) captured by GG-7 Fab preadsorbed on Petri dishes with indicated concentrations (closed bars) are compared with those between BSA-coated tips and trα₅β₁-Fc–functionalized Petri dishes (open bars) and with those between FNIII_7–10-coated tips and GG-7–coated Petri dishes without incubating with trα₅β₁-Fc (hatched bars) in the indicated cation conditions.