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. 2009 Jun 29;185(7):1275–1284. doi: 10.1083/jcb.200810002

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© 2009 Kong et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Lifetimes of FN–trα₅β₁ bonds. (A–C) Plots of lifetime (mean ± SEM) versus force of trα₅β₁-Fc–functionalized Petri dish dissociating from FNIII_7–10-coated cantilever tips (circles) in Ca²⁺/Mg²⁺ (A), Mg²⁺/EGTA (B), and Mn²⁺ (C). Data from Fig. 3 (A–C) are replotted in A–C for comparison. The lifetime versus force curves of α₅β₁-Fc–functionalized Petri dish dissociating from FNIII_7–10-coated cantilever tips are shown as triangles, and α₅β₁-Fc–coated cantilever tips dissociating from GG-7–functionalized Petri dish are shown as squares. (D) Schematic of the molecular arrangement indicating possible dissociation loci between FNIII_7–10 and trα₅β₁-Fc or between trα₅β₁-Fc and GG-7.