Figure 2.

NCE102 is required for normal plasma membrane organization. (a and b) Sur7-GFP (a) and Pma1-GFP (b) were expressed in wild-type (wt) or Δnce102 cells, and representative confocal top (bottom) and mid sections (top) are shown. (c) Wild-type and Δnce102 cells expressing Pil1-GFP (green) were pulse labeled with FM4-64 (red) for 1 min, and representative images are shown. Boxes indicate the area magnified in the bottom panels. (d) Numbers of FM4-64 intermediates per cell (n > 50) from images as in c are shown as a histogram. Bars, 5 µm.