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. 2009 Jun 29;185(7):1195–1207. doi: 10.1083/jcb.200812025

Figure 6.

Figure 6.

CCB complex depletion by α-b6C coimmunoprecipitation in BN-PAGE. Half of the digitonin-solubilized membranes from the petB-C35V strain were incubated with protein A–Sepharose beads coupled to antibodies against a peptide of cyt b6 C terminus (α-b6C). Digitonin-solubilized proteins without immunoprecipitation (−IP) and of the unbound fraction after α-b6C immunoprecipitation (+IP) were separated in the first dimension by the same BN-PAGE and in the second dimension by SDS-PAGE, and analyzed by ECL immunodetection. CCB2/CCB4/CCB3/b6 (solid boxes), CCB1/b6 (dashed boxes), and CCB2/CCB4 heterodimer (circles) are indicated.