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. 2009 Jun 29;185(7):1159–1166. doi: 10.1083/jcb.200902142

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© 2009 Peris et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Depolymerizing activity of MCAK on tyrosinated or detyrosinated MTs in lysed cells. (A) MT tyrosination/detyrosination levels in lysed cells. WT or TTL KO MEFs were lysed in a Triton X-100–based buffer, taxol stabilized, fixed, and labeled with tyrosinated (Tyr) and detyrosinated (Detyr) tubulin antibodies. WT + CPA indicates WT lysed MEFs incubated with CPA before fixation. (B) MCAK effects on lysed cells. Images of MT arrays under control conditions or after exposure to recombinant full-length MCAK. (C) Quantitative analysis of MT depolymerization in the presence of full-length MCAK or MCAK domains. The MT signal is the percentage ± SEM of the area occupied by the MT network versus total cell area. A minimum of 78 lysed cells was analyzed in each condition. ***, P < 0.001 with a t test. Bars, 50 µm.