Table III.

Information to include in the Materials and methods or figure legend

Follow the journal's instructions for authors, and/or include the following:

• Manufacturer and model of microscope

• Objective lens magnification, NA, and correction for aberration (e.g., 60x 1.4 NA Plan-Apochromat)

• Fluorescence filter set manufacturer and part number and/or the transmission and bandwidth (e.g., 490/30)

• Illumination light source (including wavelength for laser illumination)

• Camera manufacturer and model

• Software program(s) and version

• Manufacturer and model of other acquisition hardware, including confocal, filter wheels, focus motors, motorized stage, shutters, etc.

• Image acquisition settings including exposure times, gain, and binning

• Other acquisition parameters, including focus step size (for z-series), time between images (for time lapse), etc.

• Description of image processing routine used to create figures

• Description of segmentation and image analysis routine, and method of validation