Skip to main content
. 2009 Jul 13;186(1):113–128. doi: 10.1083/jcb.200811071

Figure 3.

Figure 3.

GABA-induced [Ca2+]i elevation in NG2 cells in hippocampal slices. (A) The slice was live stained with NG2 (red, anti–mouse) and then fixed by 4% paraformaldehyde for the secondary anti-NG2 immunostaining (green, anti–rabbit). The two sets of staining colocalized nicely, which excluded the possibility of nonspecific staining of live cells due to endocytosis of the antibody. The nucleus was stained by DAPI (blue). Typical NG2 cells are indicated by white arrows and arrowheads. Bar, 20 µm. (B) Confocal images of NG2 cells loaded with Fluo-4 AM (green). The NG2 cells were identified by live immunostaining with anti-NG2 (red). Note the increased [Ca2+]i in NG2 cells (indicated by arrows) during 1 mM GABA perfusion. 500 µM kynurenic acid was added to block potential secondary activation of glutamate receptors. Bar, 20 µm. (C) Time course of GABA-induced [Ca2+]i changes in NG2 cells with or without the presence of various inhibitors. (D) Summary of GABA-induced [Ca2+]i changes in NG2 cells as shown in C. Data were averaged during 55–105 s after the onset of perfusion with GABA, and normalized by the mean fluorescence intensity obtained during the control period (0–50 s before GABA perfusion) for each cell. *, P < 0.05; **, P < 0.01 compared with the corresponding control group. Error bars represent mean ± SEM.