Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jul 13;186(1):113–128. doi: 10.1083/jcb.200811071

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Tong et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — GABA-induced [Ca²⁺]_i elevation in acutely dissociated NG2 cells. (A) Images of acutely dissociated NG2 cells (arrows) loaded with the Fluo4-AM (green) and identified by live immunostaining with anti-NG2 (red). Images were taken from the same imaging field before (control) and during perfusion with 1 mM GABA. Bar, 10 µm. (B) Time course of [Ca²⁺]_i changes in dissociated NG2 cells perfusion with 1 mM GABA with or without 10 µM bicuculline. (C) Summary of GABA-induced [Ca²⁺]_i changes in dissociated NG2 cells with or without various inhibitors. Data were averaged during 20–50 s after the onset of perfusion with GABA, and normalized by the mean fluorescence intensity obtained during the control period (0–50 s before GABA perfusion) for each cell. **, P < 0.01 compared with ECS group not treated with GABA. Error bars represent mean ± SEM.