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. 2009 Jul 13;186(1):129–145. doi: 10.1083/jcb.200812150

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© 2009 Fouquet et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Epitope mapping for mAb Nc82 and genetic analysis of brp. (A) BRP fragments used for transgenic expression experiments. (B) Ectopic expression (in wing imaginal discs) of GFP-tagged BRP fragments missing either N- (D2-4) or C-terminal (D1-3) regions. Wing discs were costained for BRP^N-Term (red), BRP^Nc82 (blue), and GFP (green). The D2-4 construct shows no BRP^N-Term reactivity, whereas the D1-3 construct lacks BRP^Nc82 staining. Bar, 300 µm. (C) Genomic analysis of the brp locus. The deletion mutants (brp⁶⁹ and brp^6.1) and their parental transposon insert lines are shown in green, and the pBac transposon insert line is shown in blue. Position of EMS-induced stop codons of brp^1.3 and brp^5.45 are shown in black.