Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jul 13;186(1):85–97. doi: 10.1083/jcb.200901084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Rex et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Adenosine reverses LTP-associated actin polymerization via the A1R in a time-dependent manner. (A) F-actin was labeled in situ with Alexa Fluor 568–phalloidin in the region of physiological recording in adult hippocampal slices. Photomicrographs show densely phalloidin-labeled spines (arrows; i). Labeled spines with clear necks (arrowheads; ii) and richly labeled mushroom spines (double arrow; iii) were often detected. (B) F-actin labeling in slices receiving control stimulation (con) or TBS followed by local infusion of vehicle (veh), adenosine (Ado), or adenosine in the presence of 0.2 µM DPCPX. The plot shows the quantification (means ± SEM) of phalloidin-labeled spines for slices receiving vehicle or adenosine at the indicated minutes after TBS (*, P < 0.05 vs. control; #, P < 0.05 vs. adenosine at 0.5 min after TBS). (C, top) Representative fEPSP traces at time points enumerated in the plot. Vertical bar, 0.5 mV; horizontal bar, 5 ms. (middle) Group fEPSP slopes for slices treated with JPK (solid horizontal line). Response amplitudes were returned to predrug levels by adjusting stimulus intensity (downward arrow); the gap in the x axis represents the time to stabilize the new baseline (<10 min). Slices receiving TBS alone (upward arrow; open circles) exhibited potentiation for >40 min. Slices receiving adenosine (gray shading) beginning 30 s after TBS (closed circles) returned to potentiated levels after washout. (bottom) PPF (percent amplitudes of pulse 2 vs. pulse 1) at 50 ms (gray circles) or 100 ms (open circles) interpulse intervals in a single case from plot immediately above. (D) In situ F-actin labeling in a slice receiving TBS in the presence of JPK. The arrow indicates a densely labeled spine. (E) Adenosine was applied at 0.5, 2, or 10 min after TBS. LTP is presented as mean percentage (±SEM) of baseline for the period from 10–20 min after vehicle or adenosine washout (*, P < 0.5 vs. vehicle/TBS alone; #, P < 0.01 vs. vehicle/0.5 min after TBS). (C and E) Dashed lines indicate baseline levels. Bars: (A [i], B, and D) 5 µm; (A [ii]) 3 µm; (A [iii] and D [inset]) 2 µm.