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. 2009 Jul 13;186(1):85–97. doi: 10.1083/jcb.200901084

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© 2009 Rex et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Adenosine blocks phosphorylation of the F-actin–severing protein cofilin. Hippocampal slices receiving TBS or control stimulation to the Schaffer collaterals were processed for double pCofilin (pCof) and PSD95 immunofluorescence. 0.2 mM adenosine (Ado) or vehicle (veh) was locally applied for 4 min beginning 30 s after TBS. (A) The plot shows mean numbers (±SEM) of pCofilin/PSD95 double-labeled puncta in the zone of physiological recording (*, P < 0.05; **, P < 0.01 vs. vehicle; #, P < 0.05; ##, P < 0.01 vs. adenosine alone). (B) Results from A expressed as difference between treatment and TBS + treatment effects (TBSΔ; mean ± SEM; **, P < 0.01 vs. vehicle; #, P < 0.05 vs. adenosine). (C) Western blots from tissue treated with adenosine or vehicle for 5 min; plot of group mean (±SEM) band ODs shows that adenosine decreased pCofilin levels (*, P < 0.05). (D) Low frequency stimulation (LFS) blocked TBS-induced increases in numbers of pCofilin⁺ PSDs (mean ± SEM; *, P < 0.05 vs. control; #, P < 0.05 vs. TBS).