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. 2009 Jul 13;186(1):41–55. doi: 10.1083/jcb.200902110

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© 2009 Sengupta et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — GRASP65 must be present in opposing mitochondrial membranes. G65-GFP-ActA–transfected HeLa cells were seeded together with either mCherry-ActA (A–C) or G65-mCherry-ActA (D–F) transfected HeLa cells, and the cells were fused with polyethylene glycol. Cycloheximide was added and after 3 h the heterokaryons, without BFA treatment, were fixed and visualized. Bar, 10 µm.