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. 2009 Jul 13;186(1):41–55. doi: 10.1083/jcb.200902110

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© 2009 Sengupta et al.

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Figure 4. — Clustering by the GRASP65-binding domain of GM130. Schematic diagram of the constructs (A). 24 h after transfection, HeLa cells expressing T20-GFP (B–E), T20-GFP-GM130^Cterm (F–I), or T20-GFP-GM130^CΔ10 (J–M) were BFA treated for 30 min and analyzed using Mitotracker (red), GFP fluorescence (green), and giantin staining (blue). Bar, 10 µm. Radial profile plots (n = 3, ±SEM, >15 cells/experiment) of T20-GFP (N), T20-GFP-GM130^Cterm (O), or T20-GFP-GM130^CΔ10 (P).