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Apoptosis in Sandhoff disease mice. (a) Apoptotic cell death in thalamic nucleus of 4-month-old Sandhoff disease mouse was detected by the in situ TUNEL method. (Bar = 20 μm.) (b) Incidence of apoptotic cell death in brainstem including thalamic nuclei (BS) and spinal cord (SC) of Sandhoff disease mice. The TUNEL-positive cells were counted in semisequential coronal sections of brain and spinal cord. Data are mean ± SEM (n = 3–4).
