Table 2.

Results of CuAAC Reactions of VLP's Containing Unnatural Amino Acids, as Shown in Figure 5

entry	particlea	# incorporated per particleb	loadingc	recoveryd
1	HBV(66M)	none	0	60−70%
2	HBV(1M-N3)170−215(66M-N3)240	410−455	120 ± 15e	≤40%
3	HBV(1M-N3)170−215(M66S)	170−215	120 ± 25	60−70%
4	Qβ(K16M)	none	0	75−80%
5	Qβ(1M-N3)9−18	9−18	15 ± 3	80−85%
6	Qβ(1M-N3)9−18(K16M-N3)180	189−198	190 ± 20	75−80%
7	Qβ(1M-N3)160(K16M-N3)180	340	306 ± 30f	70−80%
8	Qβ(1M-N3)9−18(T93M-N3)180	189−198	35 ± 5	75−80%
9	Qβ(K16M-CCH)90	90	50 ± 5	20%
10	Qβ(K16M-CCH)90	90	85 ± 5g	75−80%

^aSee caption to Table 1 for nomenclature.

^bTotal number of unnatural amino acids per particle, determined by trypsin digestion and mass spectrometry as described in the text.

^cThe number of attached molecules (subscripts “m” in Figure 5). Unless otherwise indicated, the value was obtained from reaction with fluorescein reagents 3 or 10 and quantitation by calibrated UV–visible absorbance of the dye (average of at least three independent reactions).

^dAmount of purified intact capsids recovered from the reaction.

^eMore than 90% of the attachments were made at position 66.

^fUnder standard conditions; a loading of 330 ± 20 (75% recovery) was observed under the most forcing conditions tested.

^gReaction with azide 11, loading determined by quantitation of attached Se atoms by inductively coupled plasma optical emission spectroscopy.