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SDS-PAGE pattern of r-RGD-hirudin in the culture during methanol induction. M: protein marker. I0: before methanol induction. I1–7: seven samples with methanol induction for 2 days. S: wt-hirudin. It was same with wt-hirudin that the expression level of r-RGD-hirudin was more than 85%. r-RGD-hirudin was excreted into supernatant of culture
