Table 1.
Final concentration of r-RGD-hirudin (μmol/l) | Fluorescence intensity (Means ± SD) |
---|---|
0.02 | 6.27 ± 0.13 |
0.04 | 13.72 ± 1.03 |
0.09 | 16.99 ± 1.21 |
0.18 | 27.67 ± 1.57 |
0.36 | 37.11 ± 1.90 |
0.88 | 56.78 ± 2.35 |
1.78 | 57.70 ± 2.54 |
IC50 was 0.3 μmol/l which was calculated by linear correlation (Y = 12.25 + 54.18X, R = 0.96, X means final concentration of r-RGD-hirudin and Y means fluorescence intensity)