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. Author manuscript; available in PMC: 2009 Jul 20.
Published in final edited form as: Mol Cell. 2007 Jan 26;25(2):273–284. doi: 10.1016/j.molcel.2006.12.016

Figure 6. Ubiquitin binds to several SH3 domains carrying Phe at position 75.

Figure 6

(A) Binding of wildtype and F409Y SH3-3 domains to GST and GST-Ub was assayed as described for Figure 4B. (B) Alignment of the Sla1 SH3-3 domain sequence with sequences of the SH3 domains from the amphiphysins and endophilin B1. The numbering before and after the sequences refers to the protein amino acids that are the beginning and end of the indicated SH3 domains. The numbering above refers to the amino acids of Sla1. The residue highlighted in dark grey corresponds to Phe409 (Phe75) of the Sla1 SH3-3 domain. (C) Binding of amphiphysin and endophilin SH3 domains to ubiquitin. The indicated SH3 domains were expressed in E. coli and binding to GST and Ub-GST was assayed as described for Figure 2.