Figure 8.

Western blot analysis of active caspase-3 in urothelial cells on embryonic day 22 and on postnatal days 7 and 10 and in adult animals. Antibody against active caspase-3 recognizes the fragment of 17–20 kDa of a larger subunit after proteolytical cleavage of the inactive enzyme. Active form of caspase-3 is present at the position corresponding to 19 kDa on both postnatal days, but is absent on embryonic day 22 and in adult animals. Immunoblot experiments were performed twice with similar results. MW, molecular weight standards.