Table 2.
Staining results in chorioretinal sections
Oil Red O | Nile Red | BODIPY 493/503 | UC-F | EC-F | |
---|---|---|---|---|---|
Retina | − | +++ | ++ | ++ | − |
BrM | ++ | +++ | ++ | ++ | ++ |
Choroid | + | +++ | ++ | ++ | − |
BrM, Bruch's membrane; UC-F, filipin for unesterified cholesterol; EC-F, filipin for esterified cholesterol. Intensity of fluorescent staining: +++, strong; ++, medium; +, low; −, none. Large vasculature, identified within the retina and choroid in light microscopy with deferential interference contrast, was not included the evaluation of the above-mentioned structures.