Table 2.
Consensus immunoreactivity grades for each antibody at each time point
| Day
|
Weekb
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Antibodya | 1 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| Actin muscle | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Actin Sarcomeric | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Actin smooth muscle | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Amylin | 3 | n/a | n/a | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| BLA.36 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| BLA.36 equine | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a |
| Calcitonin | 3 | 3 | n/a | n/a | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a | n/a | n/a |
| Calponin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Calretinin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD117 | 3 | n/a | 3 | 2 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 1 | n/a | n/a | n/a |
| CD10 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD11d | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD18 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD1a | 3 | n/a | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a |
| CD20 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD20 equine | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a |
| CD3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD3 equine | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a |
| CD31 | 3 | 3 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD45 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD68 bovine | 3 | n/a | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a |
| CD79a | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| CD79a equine | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a |
| Chromogranin A+B | 3 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Claudin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratin 5 | 3 | 3 | 3 | 2 | 2 | 2 | 3 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratin 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratins 8/18 | 3 | 2 | 2 | 2 | 3 | 2 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratin AE1/AE3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratins HMW | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 1 | n/a | n/a | n/a | n/a | n/a | n/a |
| Cytokeratins pan | 2 | 2 | 3 | 3 | 3 | 2 | 2 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Desmin | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| E-Cadherin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Factor VIII-RA | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| GATA4 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | 3 | n/a | n/a | n/a | n/a | n/a |
| GFAP | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Glucagon | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Glut-1 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Inhibin | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a |
| Insulin | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Ki67 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Laminin | 3 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | n/a | n/a | n/a | n/a | n/a | n/a |
| Lysozyme | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| MHC II | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| MUM1 | 3 | n/a | 3 | 3 | 3 | 3 | n/a | 3 | 3 | n/a | n/a | 3 | n/a | n/a | n/a |
| MUM1 feline | 3 | n/a | n/a | 3 | 3 | 3 | 3 | 3 | 2 | 2 | 2 | 2 | n/a | n/a | n/a |
| Myel/histio antigen | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Myoglobulin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Myosin smooth muscle | 3 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Neurofilaments | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Neuron-specific enolase | 2 | 3 | 2 | 3 | 3 | 3 | 2 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| OCT-3/4 | 3 | n/a | n/a | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a |
| p63 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| PGP 9.5 | 3 | 3 | 3 | 3 | 2 | 3 | 2 | 2 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Progesterone receptor | 3 | n/a | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a |
| Prox-1 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| S-100 | 3 | 3 | 3 | 2 | 3 | 3 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Somatostatin | 2 | 3 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
| Synaptophysin | 3 | 3 | 3 | 2 | 2 | 2 | 2 | 2 | 2 | n/a | n/a | n/a | n/a | n/a | n/a |
| Tryptase | 2 | n/a | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | n/a | n/a | n/a |
| Vimentin | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | n/a | n/a | n/a | n/a | n/a | n/a |
a
All antigens were detected in canine tissues unless otherwise noted.
b
Week 1 corresponds to 7 ± 3 days. All other weeks are at 7-day intervals following week 1, except for weeks 3, 7, and 8 for GATA4 and inhibin-α, which were processed 2 days early owing to holiday schedule. Equine lymphoid tissue was processed at 4 days instead of 3 days.
GFAP, glial fibrillary acidic protein; myelo/histio antigen, myeloid/histiocytic antigen; n/a, time point was not evaluated.