Figure 1.

Ultrastructural analysis of Nanogold-labeled yeast using the Tokuyasu method to prepare cryosections. Spheroplasts obtained from the wild-type SEY6210 strain were incubated in the presence (A) or absence (B) of 4 nmol of positively charged Nanogold at 4C for 15 min before being transferred to room temperature for 30 min. After fixation, cells were processed following the Tokuyasu method adapted to yeast, and the silver enhancement reaction was performed on the obtained cryosections. Arrows indicate some of the membranes labeled with the Nanogold. ER, endoplasmic reticulum; M, mitochondria; PM, plasma membrane; V, vacuole. Bar = 200 μm.