Fig. 4.
Cryoablation plus cyclophosphamide increases IFN-γ release. Animals were divided into four groups: surgery, cyclophosphamide plus surgery, cryoablation, and cyclophosphamide plus cryoablation. They were sacrificed 10 days after local tumor removal. Splenic cell suspensions were prepared, and 1 × 106 cells were stimulated with 10 μg of AH1, the immunodominant peptide of CT26, for 6 h before surface staining with anti-mouse CD4 antibody and intracellular staining with anti-mouse IFN-γ antibody. Flow cytometric data are shown as percentage of CD4+, IFN-γ-positive cells in response to AH1 stimulation.