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HEK 293 cells stably transfected with Siglec-8 have the ability to bind 30-kDa 6′-sulfo-sLex-PAA polymer. Shown are flow cytometric histograms comparing mock-transfected HEK 293 cells that fail to bind Siglec-8 antibody and 6′-sulfo-sLex-PAA polymer. Also shown is the failure of binding of several structurally similar glycan-conjugated polymers. Results are representative of two experiments with similar results.
