Fig. 4.
The 2000-kDa 6′-sulfo-sLex PAA polymer selectively binds to eosinophils among leukocytes in whole blood. Attachment of the 2000-kDa 6′-sulfo-sLex PAA polymer and the 2C4 Siglec-8 monoclonal antibody to eosinophils was readily apparent, whereas no labeling of monocytes, lymphocytes, neutrophils, or basophils was observed. Binding of the LacNAc control PAA polymer is seen for neutrophils and a subset of lymphocytes. Results are representative of at least eight experiments with similar results.