Figure 3.

Ecdysone-dependent transcriptional upregulation of the UPS. A, B, Heat maps representing expression changes for genes involved in the ubiquitin–proteasome system. Genes in bold red print have been shown to be required for MB γ neuron pruning (Watts et al., 2003). C–E, Uba1 mRNA expression (magenta) in wt γ neurons (green) in early third-instar larvae before the ecdysone pulse that initiates pruning (C), just after the ecdysone pulse at 0 h APF (D), and in MB γ neurons at 0 h APF where EcR activity is blocked by expression of EcRDN (E). F–H, Rpn6 mRNA expression (magenta) in wt γ neurons (green) in early third-instar larvae (F), 0 h APF (G), and EcRDN-expressing γ neurons at 0 h APF (H). The panels show confocal z-projections from a 15 μm cryosection through MB neuron cell bodies. The dashed outline represents the extent of GFP-labeled MB neurons. Scale bar, 50 μm.