Figure 10.
Spatial distribution of [Ca]i sensitivity along the single cilium. (A) Fluorescent image of a single cilium and the locations of the UV laser stimuli. Stimulus intensity is depicted as a color scaling that is independently shown with a scale bar (top left column; for detail, see Takeuchi and Kurahashi, 2008). (B–G) Waveforms of the current induced by the local laser irradiation. Cell was loaded with 10 mM of caged Ca. All ROIs were circular, and diameters were 1 µm (25 pixels) for filled squares or 0.52 µm (13 pixels) for filled circles. Vh, −50 mV. 100× lens. Laser wavelength, 351 and 364 nm. Output, 70%; transmission, 100%. Data were obtained from points indicated in A. Downward transients observed immediately before the responses are artifacts caused by the trigger signals to initiate the raster scan on LSM. (H) Relation between the distance from the knob and local current responses from eight cells. 100× lens. The lowest horizontal color bars indicate the length of the cilia of corresponding colored plots.