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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 16;47(45):11725–11734. doi: 10.1021/bi801231m

Table 1.

Specific heme content and accurate mass analysis of CYP2C9 mutants

Enzyme Concentrations
 Molecular Mass
Specific content nmol/mg Expected Observed Da Δ
Wild-type 14 56669 56672 +3
F100L 11 56635 56631 −4
F114L 11 56635 56633 −2
F476L 11 56635 56630 −5
F114W 13 56708 56703 −5
F476W 12 56708 56705 −3
F114L/F476L 13 56601 56594 −7
F114W/F476W 13 56747 56741 −6