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. Author manuscript; available in PMC: 2009 Jul 20.
Published in final edited form as: Anal Biochem. 2007 Apr 2;365(2):194–200. doi: 10.1016/j.ab.2007.03.039

Figure 2.

Figure 2

A) Binding of an “8-6” RNA hairpin to poliovirus polymerase as detected by fluorescence polarization of a fluorescein label on the 5′ end of the RNA. Experiments were carried out using 10 nM RNA and the dissociation constants resulting from fitting the data to a binding isotherm are listed in Table 1. B) Gel electrophoresis analysis showing 3Dpol dependent single nucleotide elongation steps of a 32P 5′ end labeled RNA hairpin with a 3′CAG5′ templating sequence. In the presence of enzyme the RNA is elongated stepwise from a 26mer to a 29mer by the incorporation of G, U, and C, and the last lane shows that the 2nd and 3rd nucleotides are not added if the first nucleotide triphosphate (GTP) is omitted from the reaction.