Table 1.
Sequences of the self-priming RNA hairpin oligonucleotides used in the binding and elongation experiments. The RNA constructs are named with an “X-Y” convention where X is the length of the duplex region and Y is the length of the templating (boldface) single stranded 5’ extension. The fluorescein label, 
, at the end of the templating sequence is attached via a 6-carbon linker.
| Name | Kd (μM) | Sequence | Bases to be added |
|---|---|---|---|
| 8-4 | 0.250±0.01 |  |
ACUG |
| 8-6 | 0.95±0.09 |  |
AUUGCA |
| 8-8 | 1.9±0.5 |  |
AUUUUGCA |
| 8-10 | 1.20±0.02 |  |
AUUUUUUGCA |
| 4-6 | 0.69±0.02 |  |
AUUGCA |
| 6-6 | 0.30±0.01 |  |
AUUGCA |
| 8-6 | 0.95±0.09 |  |
AUUGCA |
| 12-6 | 1.3±0.2 |  |
AUUGCA |