Table 1.

Classification of Balanus amphitrite ESTs

Search method	Putative source	E-value	Annotation	N° of sequences
Blast2n vs nt				457
InterPro				438
Blast2x vs SP				458
Blast2x vs KEGG				500
			Functionally annotated	449
			Unassigned ESTs	149
			Unknown ESTs	307
	Ribosomal RNA			123
	Mitochondrial DNA			202
	Genomic DNA			580
		< e-30		191
		> e-30		407

Vector clipped ESTs longer than 50 nucleotides and with an E-value > e-5 were functionally annotated whenever possible. Blast hits with E-values < e-5 were included in the unassigned proteins. Unknown ESTs in our dataset are those with no match in sequence databases. These ESTs were considered to be of genomic DNA origin as it is unlikely that the highly conserved mitochondrial gene would not result in a Blast hit.