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. 2009 Jul 1;7:68. doi: 10.1186/1477-7827-7-68

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Copyright © 2009 Salvetti et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of caspase-3, Ki-67 and PCNA by immunohistochemistry and in situ apoptosis by TUNEL. Positive staining is shown as brown colouring of the cytoplasm/nucleus of the cells. Figures A, D, G and J correspond to healthy tertiary follicles; B, E, H and K correspond to atretic follicles and C, F, I and L correspond to cystic follicles. (A-C) caspase-3 immunolocalization, (D-F) TUNEL, (G-I) Ki-67 immunolocalization and (J-L) PCNA immunolocalization. G: Granulosa, TI: Theca Interna, TE: Theca Externa. Bars = 20 μm.