Primary antibodies |
PCNA |
PC-10. Novocastra (UK) |
Buffer Citrate pH 6. Microwave. |
1:100 |
Ki-67 |
MIB-5. Dako (Denmark) |
Buffer Citrate pH 6. Microwave. |
1:60 |
Active Caspase-3 |
Polyclonal. R&D systems (USA) |
Without antigen retrieval |
1:500 |
Bax |
Polyclonal. PU347-UP-Biogenex (San Ramon, CA, USA) |
Without antigen retrieval |
1:30 |
Bcl-2 |
Clone 100. Zymed (San Francisco, CA, USA) |
Without antigen retrieval |
1:50 |
Bcl-w |
Polyclonal. ab 13525-Abcam (Cambridge, UK) |
Without antigen retrieval |
1:100 |
Bcl-xL |
Polyclonal. ab 45002-Abcam (Cambridge, UK) |
Without antigen retrieval |
1:500 |
Secondary antibodies |
Anti-rabbit IgG |
Goat Polyclonal. 65-6140-Zymed (San Francisco, CA, USA) |
- |
1:100 |
Anti-mouse IgG |
Goat Polyclonal. AP181B Chemicon (Temecula, CA, USA) |
- |
1:100 |