Table 2.
Expression profiles of selected genes and proteins from microarray analysis, RT-PCR, immunocytochemistry and western blot analysis.
| GENE CELL | N25 | N32 | N29 | RN33B | ST14A | HIB5 | ANALYSIS |
|---|---|---|---|---|---|---|---|
| Follistatin | + | - | +/- | - | +++ | +++ | Microarray |
| + | - | - | - | +++ | ++ | RT-PCR | |
| + | +/- | - | - | +++ | +++ | Protein exp | |
| GAS6 (growth arrest specific protein 6) | +/- | +/- | +/- | +/- | +++ | +++ | Microarray |
| +/- | +/- | +/- | - | +++ | ++ | RT-PCR | |
| - | - | - | +/- | ++ | ++ | Protein exp | |
| TAPA1/CD81 | + | missing | ++ | + | +++ | +++ | Microarray |
| + | +++ | + | +/- | +++ | ++ | RT-PCR | |
| ++ | +++ | +++ | - | ++ | +++ | Protein exp | |
| AXL | missing | missing | missing | missing | missing | missing | Microarray |
| ++ | + | ++ | + | +++ | ++ | RT-PCR | |
| +++ | ++ | +++ | - | ++ | +++ | Protein exp | |
| GRO1/CXCL1 (chemokine (C-X-C motif) ligand 1) | +++ | ++ | ++ | ++ | + | + | Microarray |
| ++ | +++ | + | - | ++ | ++ | RT-PCR | |
| - | +++ | ++ | - | - | - | Protein exp | |
| TPT1 (tumor protein, translationally controlled 1) | ++ | + | ++ | +/- | +++ | +++ | Microarray |
| +++ | ++ | + | +/- | ++ | + | RT-PCR | |
| TRIM23/Arfd1 (tripartite motif-containing 23) | + | + | + | + | +++ | +++ | Microarray |
| + | + | + | ++ | +++ | ++ | RT-PCR | |
| CD82/Kai1 | ++ | ++ | +++ | + | +++ | +++ | Microarray |
| +++ | ++ | ++ | + | +++ | +++ | RT-PCR | |
| Camk2 (Ca/calmodulin dependent protein kinase typeII) | ++ | + | + | + | +++ | ++ | Microarray |
| ++ | +++ | + | + | ++ | ++ | RT-PCR | |
| Eef1a (Elongation factor 1 alpha) | ++ | + | + | + | +++ | +++ | Microarray |
| +++ | + | + | + | ++ | ++ | RT-PCR | |
The order of the scores in the table is 1) microarray, 2) RT-PCR, 3) protein analysis. The selected and compared genes were: Growth arrest specific 6 (gas6), follistatin (fst), AXL receptor tyrosine kinase (missing on the array), translationally-controlled 1 (TPT1), Kai (CD82), CD81 (Tapa-1), ADP-ribosylation factor domain protein 1 (Arfd1), Calcium/calmodulin-dependent protein kinase II (Camk2), growth regulated protein precursor CXCL1 and Eukaryotic translation elongation factor 1 alpha 1 (Eefa1). The levels of expression are compared within each method of measurement used and expressed within each group as +++ for the strongest expression measured. ++ was set for values ranging from 45–70% of the highest score (+) for 15–44% of the highest score, (+/-) for 1–14% of the highest score and (-) for no expression detected.