Figure 12. IL-17 potentiates TNF-α-induced chemokine expression in macrophages.
Primary microglia or macrophages isolated from C57BL/6 wild type mice were treated with the indicated concentrations of IL-17 or TNF-α or in combination for 24 h, whereupon CXCL2 and CCL2 production was quantitated by ELISA. Significant differences between unstimulated versus cytokine-treated cells are indicated by asterisks (**, p < 0.001), whereas significant differences between cells exposed to TNF-α versus TNF-α IL-17 are denoted by hatched signs (##, p < 0.001).