Figure 3. The frequency of CD4⁺ Th17 cells is increased in brain abscesses of TLR2 KO mice.

T cells were recovered from brain abscesses of TLR2 KO and WT mice at day 14 following S. aureus exposure using CD90-conjugated magnetic beads and stimulated with a cocktail of PMA + ionomycin for 12 h in the presence of GolgiBlock^™, whereupon IL-17 production was quantitated by intracellular cytokine staining and FACS. The results presented are representative of three independent experiments.